Terminator

Part:BBa_K4770008

Designed by: Andrea Camí Bonet   Group: iGEM23_Barcelona-UB   (2023-10-11)

TPSAD_Constitutive_Terminator

Description and Biology

Constitutive terminator. Terminator from the Photosystem I reaction center subunit II, a protein that is a component of the Chlamydomonas reinhartii’s photosystems and is found in the membrane of the thylakoid. It is a constitutive native (non-synthetic) Chlamydomonas reinhartii’s terminator and one of the strongest.

How can this part be used

In AlgaGenix, TPSAD sequence has been used for MoClo assembly as a B6 part, acting as a constitutive terminator.

Source of this part

Original sequence was obtained from MoClo kit designed by Crozet, et. al (2018)

Design considerations

MoClo kit provides you with sequences with different overhangs so you can choose the one that better fits you design/cloning plan.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None